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Molecular cloning, expression, purification and characterization of a novel cellulase gene (Bh-EGaseI) in the beetle Batocera horsfieldi.

Gene. 2016-01; 
MeiHui-Zhen,XiaDing-Guo,ZhaoQiao-Ling,ZhangGuo-Zheng,QiuZhi-Yong,QianPing,LuC
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Monoclonal Antibody Services … at room temperature. After three 10 min washes with TBST, the blocked membrane was incubated with anti-His monoclonal antibody [1:10,000 (v/v); GenScript, USA] on a shaker for 1 h at room temperature. The membrane was … Get A Quote

摘要

Wood-feeding insects depend heavily on the secretion of a combination of cellulases, mainly endoglucanases and other glucanases such as exoglucanases and xylanases, to achieve efficient digestion of the cellulose of cellulosic materials. In this paper, we report a novel cellulose Bh-EGaseI belonging to the glycoside hydrolase family 45(gh45-1) obtained from the beetle Batocera horsfieldi. The Bh-EGaseI gene spans 714 bp and consists of three exons coding 237 amino acid residues. The cDNA encoding Bh-EGaseI was expressed as 25 KDa in baculovirus-infected Bombyx mori larvae. The expression products of Bh-EGaseI from larval hemolymph showed a specific enzymatic activity of approximately 1030.87 IU per mg. Th... More

关键词

Baculovirus,Batocera horsfieldi,Cellulase,Endoglucanases,Silk