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Detection of the circulating antigen 14-3-3 protein of Schistosoma japonicum by time-resolved fluoroimmunoassay in rabbits.

Parasit Vectors. 2011; 
QianChun-Yan，HuangBiao，YuChuan-Xin，ZhangJue，YinXu-Ren，WangJie，SongLi-Jun，ZhangWei，KeXue
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Biochemicals 100 μl of horseradish peroxidase (HRP)-conjugated 5C6 solution diluted in PBST was added and incubated at 37°C for 1 h. The plate was washed as above and the reaction was visualized by adding 100 μl of TMB substrate solution (GenScript Get A Quote

摘要

Schistosomiasis remains a major public health concern that afflicts millions of people worldwide. Low levels of Schistosoma infection require more sensitive diagnostic methods. In this study， a time-resolved fluoroimmunoassay (TRFIA) was developed for detecting the signal transduction protein 14-3-3， a circulating antigen of Schistosoma japonicum.

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