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Recombinant production of active Streptococcus pneumoniae CysE in E. coli facilitated by codon optimized BL21(DE3)-RIL and detergent.

Prep. Biochem. Biotechnol.. 2019; 
VermaDeepali,AntilMonika,GuptaV
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Bacterial Expression System the percentage of rare codons in Spn cysE when expressed in heterologous E. coli host was deter- mined by codon Usage Analyzer 2.1 and GenScript Rare Codon Analysis Tool. The former calculates … " https://www.tandfonline.com/doi/abs/10.1080/10826068.2019.1573194 Get A Quote

摘要

The emergence of drug resistance in Streptococcus pneumoniae (Spn) is a global health threat and necessitates discovery of novel therapeutics. The serine acetyltransferase (also known as CysE) is an enzyme of cysteine biosynthesis pathway and is reported to be essential for the survival of several pathogenic bacteria. Therefore, it appears to be a very attractive target for structure-function understanding and inhibitor design. This study describes the molecular cloning of cysE from Spn in the pET21c vector and efforts carried out for expression and purification of active recombinant CysE. Significant expression of recombinant Spn cysE could be achieved in codon optimized BL21(DE3)-RIL strain as opposed to co... More

关键词

,BL21(DE3)-RIL,Codon adaptation index,CysE or SAT,Triton X-100,expression,purifica