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High-resolution melting analysis of HPV-16L1 gene methylation: A promising method for prognosing cervical cancer.

Clin. Biochem.. 2015;

QiuCui，ZhiYanfang，ShenYong，GongJiaomei，LiYa，LiXi

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	The completely methylated and unmethylated HPV-16 L1 gene standards were synthesized (with additional cloning into E.coli) according to the genetic base sequence of the target gene (Genscript, Nanjing, China).	Get A Quote

摘要

Methylation-sensitive high-resolution melting (MS-HRM) is a new technique for DNA methylation analysis， but it is rarely used for the detection of viral DNA methylation. In this study， we investigated the HPV-16L1 gene methylation that is detected by MS-HRM as a potential biomarker for prognosing cervical dysplasia and cancer.