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MiR-301a promotes embryonic stem cell differentiation to cardiomyocytes.

World J Stem Cells. 2019; 
Zhen LX, Gu YY, Zhao Q, Zhu HF, Lv JH, Li SJ, Xu Z, Li L, Yu ZR.
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Custom DNA/RNA Oligos … All cells were cultured at 37 °C in a 5% CO 2 environment unless stated otherwise. Oligos and transfection. All primers and miR-301a mimic and negative control oligos were synthesized by GenScript (Nanjing, China). Forward … Get A Quote

摘要

Cardiovascular disease is the leading cause of death worldwide. Tissue repair after pathological injury in the heart remains a major challenge due to the limited regenerative ability of cardiomyocytes in adults. Stem cell-derived cardiomyocytes provide a promising source for the cell transplantation-based treatment of injured hearts.,To explore the function and mechanisms of miR-301a in regulating cardiomyocyte differentiation of mouse embryonic stem (mES) cells, and provide experimental evidence for applying miR-301a to the cardiomyocyte differentiation induction from stem cells.,mES cells with or without overexpression of miR-301a were applied for all functional assays. The hanging drop technique was applied ... More

关键词

Cardiomyocytes; Differentiation; Mouse embryonic stem cells; miR-301a