至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

An isothermal strand displacement amplification strategy for nucleic acids using junction forming probes and colorimetric detection

springerlink. 2017; 
Xuchu Wang, Weiwei Liu, Binbin Yin, Yiwen Sang, Zhenping Liu, Yu Dai, Xiuzhi Duan, Gong Zhang, Shijia Ding & Zhihua Tao
Products/Services Used Details Operation
Molecular Biology Tools … DEPC-treated water was obtained from Shanghai Sangon Biological Engineering Technology & Services (Shanghai, China wwwsangoncom) and used in all experiments Hemin was obtained from GenScript Biotech Co, Ltd … Get A Quote

摘要

The authors describe a method for DNA target recognition and signal amplification that is based on the target-induced formation of a three way junction. The subsequent assembly of two DNA probes releases the inhibitory strand and triggers a downstream strand displacement amplification. This causes the formation of a G-rich single sequence that binds to a hemin monomer with its peroxidase-mimicking properties. The resulting peroxidase (POx) activity is quantified by using H2O2 and TMB as the substrate. In the presence of an inhibitor, in contrast, the POx-like activity is strongly reduced. This forms the basis for a highly sensitive DNA assay. It has a 0.8 pM detection limit when operated at a wavelength of 45... More

关键词