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miR-550a-5p Functions as a Tumor Promoter by Targeting LIMD1 in Lung Adenocarcinoma

Front Oncol. 2020; 
Zi-Zhang Guo, Zi-Jian Ma, Yao-Zhou He, Wei Jiang, Yang Xia, Chun-Feng Pan, Ke Wei, Yi-Jun Shi, Liang Chen, Yi-Jiang Chen
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Mutagenesis Services … Dual-Luciferase Assay. The 3′-UTR sequence or the mutant sequence of LIMD1 was cloned into pGL3 promoter vector (Genscript, Nanjing, China). A549 cells were cultured in 24-well plates transfected miR-550a-5p mimics or negative control (NC) … Get A Quote

摘要

Lung adenocarcinoma accounts for half of all lung cancer cases in most countries. Mounting evidence has demonstrated that microRNAs play important roles in cancer progression, and some of them can be identified as potential biomarkers. This study aimed to explore the role of miR-550a-5p, a lung adenocarcinoma-associated mature microRNA screened out from the TCGA database via R-studio and Perl, with abundant expression in samples and with 5-year survival prognosis difference, as well as having not been studied in lung cancer yet. Potential target genes were predicted by the online database. Gene ontology enrichment, pathway enrichment, protein-protein interaction network, and hub genes-microRNA network were cons... More

关键词

cancer therapy, cell proliferation, lung adenocarcinoma, microRNA, oncogene