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Induction of EBV latent membrane protein-2A (LMP2A)-specific T cells and construction of individualized TCR-engineered T cells for EBV-associated malignancies

J Immunother Cancer. 2021-07; 
Chaoting Zhang, Qin Tan, Shance Li, Luyan Shen, Jingtao Zhang, Ying Liu, Wenjun Yang, Zheming Lu
Products/Services Used Details Operation
Custom Vector Construction … TCRα/β chains were synthesized (GenScript) and cloned into our lentivirus vector.TCRs were constructed in a β-α chain order and their constant regions were replaced by mouse counterparts modified with hydrophobic substitution and added disulfide bond as previously … Get A Quote

摘要

background: Latent membrane protein-2A (LMP2A)-specific TCR-engineered T cells could be a promising treatment approach to Epstein-Barr virus-associated malignancies. However, previous studies mainly reported LMP2A-reactive TCRs only focusing on specific HLA subtypes and corresponding epitopes, and thus, they were only suitable for patients with specific HLA. methods: Due to hugely varied HLA subtypes and presented LMP2A epitopes in different individuals, our study attempted to develop an individualized approach, based on the weekly in vitro stimulation of peripheral T cells for 2 weeks with autologous dendritic cells (DCs) pulsed with a pool of LMP2A peptides covering LMP2A whole protein and combination analy... More

关键词

adaptive immunity, adoptive, immunotherapy