Products/Services Used | Details | Operation |
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Mutant Libraries> | … containing the open reading frames encoding two mutants, SS2-des116–120 and SS2-des119–126, have been designed and produced by GenScript Biotech (Leiden, Netherlands) … | Get A Quote |
The superfamily of vertebrate ribonucleases, a large group of evolutionarily related proteins, continues to provide interesting structural and functional information. In particular, the crystal structure of SS-RNase-2 from Salmo salar (SS2), here presented, has revealed a novel auto-inhibition mechanism that enriches the number of inhibition strategies observed in some members of the family. Within an essentially unmodified RNase folding, the SS2 active site cleft is in part obstructed by the collapse of an extra pentapeptide inserted in the C-terminal region. This unexpected intrusion alters the organization of the catalytic triad by pushing one catalytic histidine off the pocket. Possible mechanisms to remove... More