Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | … For the immunoprecipitation, 2.5 μg of mouse monoclonal anti-his antibody was used (GenScript Biotech, Piscataway, NJ, USA). Following coimmunoprecipitation, the samples were … | Get A Quote |
In nature, dynamic interactions between enzymes play a crucial role in defining cellular metabolism. By controlling the spatial and temporal organization of these supramolecular complexes called metabolons, natural metabolism can be tuned in a highly dynamic manner. Here, we repurpose the CRISPR-Cas6 family proteins as a synthetic strategy to create dynamic metabolons by combining the ease of RNA processing and the predictability of RNA hybridization for protein assembly. By disturbing RNA-RNA networks using toehold-mediated strand displacement reactions, on-demand assembly and disassembly are achieved using both synthetic RNA triggers and mCherry messenger RNA. Both direct and 'Turn-On' assembly of the pathway... More