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Novel Bacterial Production of Two Different Bioactive Forms of Human Stem-Cell Factor

Authors: Eunyoung Lee, Michelle Novais de Paula, Sangki Baek, Huynh Kim Khanh Ta, Minh Tan Nguyen, Taeck-Hyun Jeong, Chong Jai Kim, Yeon Jin Jang, Han Choe

Journal: Int J Mol Sci

PubMed ID: 34198626

Doi: 10.3390/ijms22126361

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The small G-protein RalA promotes progression and metastasis of triple-negative breast cancer

Authors: Katie A Thies, Matthew W Cole, Rachel E Schafer, Jonathan M Spehar, Dillon S Richardson, Sarah A Steck, Manjusri Das, Arthur W Lian, Alo Ray, Reena Shakya, Sue E Knoblaugh, Cynthia D Timmers, Michael C Ostrowski, Arnab Chakravarti, Gina M Sizemore, Steven T Sizemore

Journal: Breast Cancer Res

PubMed ID: 34118960

Doi: 10.1186/s13058-021-01438-3

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A role for the Cockayne Syndrome B (CSB)-Elongin ubiquitin ligase complex in signal-dependent RNA polymerase II transcription

Authors: Juston C Weems, Brian D Slaughter, Jay R Unruh, Kyle J Weaver, Brandon D Miller, Kym M Delventhal, Joan W Conaway, Ronald C Conaway

Journal: J Biol Chem

PubMed ID: 34116057

Doi: 10.1016/j.jbc.2021.100862

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Machine learning analyses of antibody somatic mutations predict immunoglobulin light chain toxicity

Authors: Maura Garofalo, Luca Piccoli, Margherita Romeo, Maria Monica Barzago, Sara Ravasio, Mathilde Foglierini, Milos Matkovic, Jacopo Sgrignani, Raoul De Gasparo, Marco Prunotto, Luca Varani, Luisa Diomede, Olivier Michielin, Antonio Lanzavecchia, Andrea Cavalli

Journal: Nat Commun

PubMed ID: 34112780

Doi: 10.1038/s41467-021-23880-9

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Prdx1 Interacts with ASK1 upon Exposure to HO and Independently of a Scaffolding Protein

Authors: Trung Nghia Vo, Julia Malo Pueyo, Khadija Wahni, Daria Ezeriņa, Jesalyn Bolduc, Joris Messens

Journal: Antioxidants (Basel)

PubMed ID: 34209102

Doi: 10.3390/antiox10071060

Products & Services: Mammalian Expression

Structural basis for SARS-CoV-2 envelope protein recognition of human cell junction protein PALS1

Authors: Jin Chai, Yuanheng Cai, Changxu Pang, Liguo Wang, Sean McSweeney, John Shanklin, Qun Liu

Journal: Nat Commun

PubMed ID: 34103506

Doi: 10.1038/s41467-021-23533-x

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Novel Bisubstrate Inhibitors for Protein N-Terminal Acetyltransferase D

Authors: Youchao Deng, Sunbin Deng, Yi-Hsun Ho, Sarah M Gardner, Zhi Huang, Ronen Marmorstein, Rong Huang

Journal: J Med Chem

PubMed ID: 34110812

Doi: 10.1021/acs.jmedchem.1c00141

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The Bone Regeneration Capacity of BMP-2 + MMP-10 Loaded Scaffolds Depends on the Tissue Status

Authors: Patricia Garcia-Garcia, Ricardo Reyes, José Antonio Rodriguez, Tomas Martín, Carmen Evora, Patricia Díaz-Rodríguez, Araceli Delgado

Journal: Pharmaceutics

PubMed ID: 34209593

Doi: 10.3390/pharmaceutics13070979

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CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression

Authors: Yi Liu, Simin Chen, Gang Peng, Yiwei Liao, Xuegong Fan, Zuping Zhang, Chenfu Shen

Journal: Cancer Cell Int

PubMed ID: 34112159

Doi: 10.1186/s12935-021-02001-y

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ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion

Authors: Qiangmin Zhang, Julia Gefter, W Bruce Sneddon, Tatyana Mamonova, Peter A Friedman

Journal: iScience

PubMed ID: 34189428

Doi: 10.1016/j.isci.2021.102770

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A liposome-displayed hemagglutinin vaccine platform protects mice and ferrets from heterologous influenza virus challenge

Authors: Zachary R Sia, Xuedan He, Ali Zhang, Jann C Ang, Shuai Shao, Amal Seffouh, Wei-Chiao Huang, Michael R D'Agostino, Amir Teimouri Dereshgi, Sambhara Suryaprakash, Joaquin Ortega, Hanne Andersen, Matthew S Miller, Bruce A Davidson, Jonathan F Lovell

Journal: Proc Natl Acad Sci U S A

PubMed ID: 34050027

Doi: 10.1073/pnas.2025759118

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Surface-Shaving Proteomics of Mycobacterium marinum Identifies Biofilm Subtype-Specific Changes Affecting Virulence, Tolerance, and Persistence

Authors: Kirsi Savijoki, Henna Myllymäki, Hanna Luukinen, Lauri Paulamäki, Leena-Maija Vanha-Aho, Aleksandra Svorjova, Ilkka Miettinen, Adyary Fallarero, Teemu O Ihalainen, Jari Yli-Kauhaluoma, Tuula A Nyman, Mataleena Parikka

Journal: mSystems

PubMed ID: 34156290

Doi: 10.1128/mSystems.00500-21

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LINC_00355 promotes gastric cancer progression by upregulating PHF19 expression through sponging miR-15a-5p

Authors: Jishui Zhang, Wenhao Lv, Yagang Liu, Weihua Fu, Baosheng Chen, Qiutong Ma, Xin Gao

Journal: BMC Cancer

PubMed ID: 34078310

Doi: 10.1186/s12885-021-08227-3

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Significant Inactivation of SARS-CoV-2 In Vitro by a Green Tea Catechin, a Catechin-Derivative, and Black Tea Galloylated Theaflavins

Authors: Eriko Ohgitani, Masaharu Shin-Ya, Masaki Ichitani, Makoto Kobayashi, Takanobu Takihara, Masaya Kawamoto, Hitoshi Kinugasa, Osam Mazda

Journal: Molecules

PubMed ID: 34208050

Doi: 10.3390/molecules26123572

Products & Services: Recombinant Proteins

A novel mouse AAV6 hACE2 transduction model of wild-type SARS-CoV-2 infection studied using synDNA immunogens

Authors: Ebony N Gary, Bryce M Warner, Elizabeth M Parzych, Bryan D Griffin, Xizhou Zhu, Nikesh Tailor, Nicholas J Tursi, Mable Chan, Mansi Purwar, Robert Vendramelli, Jihae Choi, Kathy L Frost, Sophia Reeder, Kevin Liaw, Edgar Tello, Ali R Ali, Kun Yun, Yanlong Pei, Sylvia P Thomas, Amira D Rghei, Matthew M Guilleman, Kar Muthumani, Trevor Smith, Sarah K Wootton, Ami Patel, David B Weiner, Darwyn Kobasa

Journal: iScience

PubMed ID: 34124612

Doi: 10.1016/j.isci.2021.102699

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The ORF8 protein of SARS-CoV-2 mediates immune evasion through down-regulating MHC-Ι

Authors: Yiwen Zhang, Yingshi Chen, Yuzhuang Li, Feng Huang, Baohong Luo, Yaochang Yuan, Baijin Xia, Xiancai Ma, Tao Yang, Fei Yu, Jun Liu, Bingfeng Liu, Zheng Song, Jingliang Chen, Shumei Yan, Liyang Wu, Ting Pan, Xu Zhang, Rong Li, Wenjing Huang, Xin He, Fei Xiao, Junsong Zhang, Hui Zhang

Journal: Proc Natl Acad Sci U S A

PubMed ID: 34021074

Doi: 10.1073/pnas.2024202118

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Evolution of KIPPIS as a versatile platform for evaluating intracellularly functional peptide aptamers

Authors: Daiki Kashima, Masahiro Kawahara

Journal: Sci Rep

PubMed ID: 34083659

Doi: 10.1038/s41598-021-91287-z

Products & Services: PCR Cloning and Subcloning

Aneuploid senescent cells activate NF-κB to promote their immune clearance by NK cells

Authors: Ruoxi W Wang, Sonia Viganò, Uri Ben-David, Angelika Amon, Stefano Santaguida

Journal: EMBO Rep

PubMed ID: 34105235

Doi: 10.15252/embr.202052032

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The Functional Consequences of the Novel Ribosomal Pausing Site in SARS-CoV-2 Spike Glycoprotein RNA

Authors: Olga A Postnikova, Sheetal Uppal, Weiliang Huang, Maureen A Kane, Rafael Villasmil, Igor B Rogozin, Eugenia Poliakov, T Michael Redmond

Journal: Int J Mol Sci

PubMed ID: 34204305

Doi: 10.3390/ijms22126490

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Dynamics of alternative splicing during somatic cell reprogramming reveals functions for RNA-binding proteins CPSF3, hnRNP UL1, and TIA1

Authors: Claudia Vivori, Panagiotis Papasaikas, Ralph Stadhouders, Bruno Di Stefano, Anna Ribó Rubio, Clara Berenguer Balaguer, Serena Generoso, Anna Mallol, José Luis Sardina, Bernhard Payer, Thomas Graf, Juan Valcárcel

Journal: Genome Biol

PubMed ID: 34082786

Doi: 10.1186/s13059-021-02372-5

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Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell

Authors: Maritza Puray-Chavez, Kyle M LaPak, Travis P Schrank, Jennifer L Elliott, Dhaval P Bhatt, Megan J Agajanian, Ria Jasuja, Dana Q Lawson, Keanu Davis, Paul W Rothlauf, Zhuoming Liu, Heejoon Jo, Nakyung Lee, Kasyap Tenneti, Jenna E Eschbach, Christian Shema Mugisha, Emily M Cousins, Erica W Cloer, Hung R Vuong, Laura A VanBlargan, Adam L Bailey, Pavlo Gilchuk, James E Crowe, Michael S Diamond, D Neil Hayes, Sean P J Whelan, Amjad Horani, Steven L Brody, Dennis Goldfarb, M Ben Major, Sebla B Kutluay

Journal: Cell Rep

PubMed ID: 34214467

Doi: 10.1016/j.celrep.2021.109364

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Effective Killing of Acute Myeloid Leukemia by TIM-3 Targeted Chimeric Antigen Receptor T Cells

Authors: Wen-Hsin Sandy Lee, Zhiyong Ye, Alice M S Cheung, Y P Sharon Goh, Hsueh Ling Janice Oh, Ravisankar Rajarethinam, Siok Ping Yeo, Mun Kuen Soh, Esther Hian Li Chan, Lip Kun Tan, Soo-Yong Tan, Charles Chuah, Wee Joo Chng, John E Connolly, Cheng-I Wang

Journal: Mol Cancer Ther

PubMed ID: 34158344

Doi: 10.1158/1535-7163.MCT-20-0155

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Design, synthesis and in vitro biological evaluation of a novel class of anti-adenovirus agents based on 3-amino-1,2-propanediol

Authors: Sarah Mazzotta, Judith Berastegui-Cabrera, Margarita Vega-Holm, María Del Rosario García-Lozano, Marta Carretero-Ledesma, Francesca Aiello, José Manuel Vega-Pérez, Jerónimo Pachón, Fernando Iglesias-Guerra, Javier Sánchez-Céspedes

Journal: Bioorg Chem

PubMed ID: 34175724

Doi: 10.1016/j.bioorg.2021.105095

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In vitro reconstitution of Sgk3 activation by phosphatidylinositol 3-phosphate

Authors: Daniel Pokorny, Linda Truebestein, Kaelin D Fleming, John E Burke, Thomas A Leonard

Journal: J Biol Chem

PubMed ID: 34181950

Doi: 10.1016/j.jbc.2021.100919

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A phase transition enhances the catalytic activity of SARM1, an NAD glycohydrolase involved in neurodegeneration

Authors: Heather S Loring, Victoria L Czech, Janneke D Icso, Lauren O'Connor, Sangram S Parelkar, Alexandra B Byrne, Paul R Thompson

Journal: Elife

PubMed ID: 34184985

Doi: 10.7554/eLife.66694

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Puma, noxa, p53, and p63 differentially mediate stress pathway induced apoptosis

Authors: Jun Wang, Holly R Thomas, Zhang Li, Nan Cher Florence Yeo, Hannah E Scott, Nghi Dang, Mohammed Iqbal Hossain, Shaida A Andrabi, John M Parant

Journal: Cell Death Dis

PubMed ID: 34193827

Doi: 10.1038/s41419-021-03902-6

Products & Services: PCR and Cloning

Structural basis of mechano-chemical coupling by the mitotic kinesin KIF14

Authors: Matthieu P M H Benoit, Ana B Asenjo, Mohammadjavad Paydar, Sabin Dhakal, Benjamin H Kwok, Hernando Sosa

Journal: Nat Commun

PubMed ID: 34131133

Doi: 10.1038/s41467-021-23581-3

Products & Services: Proteins, Expression, Isolation and Analysis

Assembly principles and stoichiometry of a complete human kinetochore module

Authors: Kai Walstein, Arsen Petrovic, Dongqing Pan, Birte Hagemeier, Dorothee Vogt, Ingrid R Vetter, Andrea Musacchio

Journal: Sci Adv

PubMed ID: 34193424

Doi: 10.1126/sciadv.abg1037

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Synthetic extracellular matrices with tailored adhesiveness and degradability support lumen formation during angiogenic sprouting

Authors: Jifeng Liu, Hongyan Long, Dagmar Zeuschner, Andreas F B Räder, William J Polacheck, Horst Kessler, Lydia Sorokin, Britta Trappmann

Journal: Nat Commun

PubMed ID: 34099677

Doi: 10.1038/s41467-021-23644-5

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Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota

Authors: Djalma S Lima-Junior, Siddharth R Krishnamurthy, Nicolas Bouladoux, Nicholas Collins, Seong-Ji Han, Erin Y Chen, Michael G Constantinides, Verena M Link, Ai Ing Lim, Michel Enamorado, Christophe Cataisson, Louis Gil, Indira Rao, Taylor K Farley, Galina Koroleva, Jan Attig, Stuart H Yuspa, Michael A Fischbach, George Kassiotis, Yasmine Belkaid

Journal: Cell

PubMed ID: 34166614

Doi: 10.1016/j.cell.2021.05.020

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Directed evolution of AAV accounting for long-term and enhanced transduction of cardiovascular endothelial cells

Authors: Y B Liu, B C Xu, Y T Chen, X Yuan, J Y Liu, T Liu, G Z Du, W Jiang, Y Yang, Y Zhu, L J Chen, B S Ding, Y Q Wei, L Yang

Journal: Mol Ther Methods Clin Dev

PubMed ID: 34485601

Doi: 10.1016/j.omtm.2021.05.015

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A single dose of the SARS-CoV-2 vaccine BNT162b2 elicits Fc-mediated antibody effector functions and T cell responses

Authors: Alexandra Tauzin, Manon Nayrac, Mehdi Benlarbi, Shang Yu Gong, Romain Gasser, Guillaume Beaudoin-Bussières, Nathalie Brassard, Annemarie Laumaea, Dani Vézina, Jérémie Prévost, Sai Priya Anand, Catherine Bourassa, Gabrielle Gendron-Lepage, Halima Medjahed, Guillaume Goyette, Julia Niessl, Olivier Tastet, Laurie Gokool, Chantal Morrisseau, Pascale Arlotto, Leonidas Stamatatos, Andrew T McGuire, Catherine Larochelle, Pradeep Uchil, Maolin Lu, Walther Mothes, Gaston De Serres, Sandrine Moreira, Michel Roger, Jonathan Richard, Valérie Martel-Laferrière, Ralf Duerr, Cécile Tremblay, Daniel E Kaufmann, Andrés Finzi

Journal: Cell Host Microbe

PubMed ID: 34133950

Doi: 10.1016/j.chom.2021.06.001

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Absolute protein assay for the simultaneous quantification of two epoxide hydrolases in rats by mass spectrometry-based targeted proteomics

Authors: Ting Wu, Xiaoyun Xi, Ying Chen, Chao Jiang, Qian Zhang, Guoliang Dai, Yongtao Bai, Weidong Zhang, Ting Ni, Jiandong Zou, Wenzheng Ju, Meijuan Xu

Journal: J Sep Sci

PubMed ID: 34008891

Doi: 10.1002/jssc.202100066

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Sphingomonas sp. KT‑1 PahZ2 Structure Reveals a Role for Conformational Dynamics in Peptide Bond Hydrolysis

Authors: Chad A. BrambleyChad A. Brambley Department of Chemistry, Middle Tennessee State University, 1301 East Main Street, Murfreesboro, Tennessee 37132, United States More by Chad A. Brambley , Tarah J. Yared, Marriah Gonzalez, Amanda L. Jansch, Jamie R. Wallen, Mitch H. Weiland*, and Justin M. Miller*

Journal: The Journal of Physical Chemistry B

PubMed ID: 34060838

Doi: doi.org/10.1021/acs.jpcb.1c01216

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Molecular basis of anticoagulant and anticomplement activity of the tick salivary protein Salp14 and its homologs

Authors: Stepan S. Denisov, Johannes H. Ippel, Elisabetta Castoldi, Ben J. Mans, Tilman M. Hackeng, and Ingrid Dijkgraaf

Journal: Journal of Biological Chemistry

PubMed ID: 34118237

Doi: 10.1016/j.jbc.2021.100865

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Phosphatidylserine externalized on the colonic capillaries as a novel pharmacological target for IBD therapy

Authors: Xuerui Zhang, Lulu Song, Lin Li, Banghui Zhu, Lina Huo, Zhaoqing Hu, Xinran Wang, Jie Wang, Mengyue Gao, Jing Zhang and Zichun Hua

Journal: Signal transduction and targeted therapy

PubMed ID: 34131110

Doi: 10.1038/s41392-021-00626-z

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Aggrecan modulates the expression and phosphorylation of tau in a novel bigenic TauP301L - Acan mouse model

Authors: Schmidt S,Stapf C,Schmutzler S,Lachmann I,Arendt T,Holzer M,Sonntag M,Morawski M

Journal: The European journal of neuroscience

PubMed ID: 32737917

Doi: 10.1111/ejn.14923

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A sensitive and reproducible cell-based assay via secNanoLuc to detect neutralizing antibody against adeno-associated virus vector capsid

Authors: Nemekhbayar Baatartsogt, Yuji Kashiwakura, Morisada Hayakawa, Nobuhiko Kamoshita, Takafumi Hiramoto, Hiroaki Mizukami, Tsukasa Ohmori

Journal: Mol Ther Methods Clin Dev

PubMed ID: 34485602

Doi: 10.1016/j.omtm.2021.06.004

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Circular RNA TLK1 Promotes Sepsis-Associated Acute Kidney Injury by Regulating Inflammation and Oxidative Stress Through miR-106a-5p/HMGB1 Axis

Authors: Hai-Ping Xu, Xiao-Ying Ma, Chen Yang

Journal: Front Mol Biosci

PubMed ID: 34250012

Doi: 10.3389/fmolb.2021.660269

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High-resolution structure of the vWF A1 domain in complex with caplacizumab, the first nanobody-based medicine for treating acquired TTP

Authors: Hyun Tae Lee, Ui Beom Park, Tae Jun Jeong, Nahyeon Gu, Sang Hyung Lee, Yujin Kim, Yong-Seok Heo

Journal: Biochem Biophys Res Commun

PubMed ID: 34144500

Doi: 10.1016/j.bbrc.2021.06.030

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Delayed KCNQ1/KCNE1 assembly on the cell surface helps I fulfil its function as a repolarization reserve in the heart

Authors: Zachary T Wilson, Min Jiang, Jing Geng, Sukhleen Kaur, Samuel W Workman, Jon Hao, Tytus Bernas, Gea-Ny Tseng

Journal: J Physiol

PubMed ID: 33963564

Doi: 10.1113/JP281773

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Antibody Levels to Plasmodium falciparum Erythrocyte Membrane Protein 1-DBLγ11 and DBLδ-1 Predict Reduction in Parasite Density

Authors: Brittany N Araj, Bruce Swihart, Robert Morrison, Patricia Gonzales Hurtado, Andrew Teo, Almahamoudou Mahamar, Oumar Attaher, Bacary S Diarra, Santara Gaoussou, Djibrilla Issiaka, Alassane Dicko, Patrick E Duffy, Michal Fried

Journal: mSystems

PubMed ID: 34128693

Doi: 10.1128/mSystems.00347-21

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Design of a Near Infrared Fluorescent Ureter Imaging Agent for Prevention of Ureter Damage during Abdominal Surgeries

Authors: Sakkarapalayam M Mahalingam, Karson S Putt, Madduri Srinivasarao, Philip S Low

Journal: Molecules

PubMed ID: 34205289

Doi: 10.3390/molecules26123739

Products & Services: Proteins, Expression, Isolation and Analysis

Structure, immunogenicity, and conformation-dependent receptor binding of the post-fusion human metapneumovirus F protein

Authors: Jiachen Huang, Pradeep Chopra, Lin Liu, Tamas Nagy, Jackelyn Murray, Ralph A Tripp, Geert-Jan Boons, Jarrod J Mousa

Journal: J Virol

PubMed ID: 34160259

Doi: 10.1128/JVI.00593-21

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SARS-CoV-2-specific CD8 T-cell responses and TCR signatures in the context of a prominent HLA-A*24:02 allomorph

Authors: Louise C Rowntree, Jan Petersen, Jennifer A Juno, Priyanka Chaurasia, Kathleen Wragg, Marios Koutsakos, Luca Hensen, Adam K Wheatley, Stephen J Kent, Jamie Rossjohn, Katherine Kedzierska, Thi Ho Nguyen

Journal: Immunol Cell Biol

PubMed ID: 34086357

Doi: 10.1111/imcb.12482

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Recruitment of phospholipase Cγ1 to the non-structural membrane protein pK15 of Kaposi Sarcoma-associated herpesvirus promotes its Src-dependent phosphorylation

Authors: Naira Samarina, George Ssebyatika, Tanvi Tikla, Ja-Yun Waldmann, Bizunesh Abere, Vittoria Nanna, Michelangelo Marasco, Teresa Carlomagno, Thomas Krey, Thomas F Schulz

Journal: PLoS Pathog

PubMed ID: 34143834

Doi: 10.1371/journal.ppat.1009635

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Detection of antibodies neutralizing historical and emerging SARS-CoV-2 strains using a thermodynamically coupled de novo biosensor system

Authors: Jason Z Zhang, Hsien-Wei Yeh, Alexandra C Walls, Basile I M Wicky, Kaiti Sprouse, Laura A VanBlargan, Rebecca Treger, Alfredo Quijano-Rubio, Minh N Pham, John C Kraft, Ian C Haydon, Wei Yang, Michelle DeWitt, Cameron Chow, Lauren Carter, Mark H Wener, Lance Stewart, David Veesler, Michael S Diamond, David Baker

Journal: biorxiv

PubMed ID: 34189528

Doi: 10.1101/2021.06.22.449355

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Canine Transforming Growth Factor-β Receptor 2-Ig: A Potential Candidate Biologic for Melanoma Treatment That Reverses Transforming Growth Factor-β1 Immunosuppression

Authors: Hiroto Takeuchi, Satoru Konnai, Naoya Maekawa, Satoshi Takagi, Hiroshi Ohta, Noboru Sasaki, Sangho Kim, Tomohiro Okagawa, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi

Journal: Front Vet Sci

PubMed ID: 34195245

Doi: 10.3389/fvets.2021.656715

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The protective effect of HOXA5 on carotid atherosclerosis occurs by modulating the vascular smooth muscle cell phenotype

Authors: Yuchen Jing, Bai Gao, Zhiyang Han, Lifang Xia, Shijie Xin

Journal: Mol Cell Endocrinol

PubMed ID: 34126188

Doi: 10.1016/j.mce.2021.111366

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Tripterygium glycoside suppresses epithelial‑to‑mesenchymal transition of diabetic kidney disease podocytes by targeting autophagy through the mTOR/Twist1 pathway

Authors: Mei Tao, Danna Zheng, Xudong Liang, Diandian Wu, Kang Hu, Juan Jin, Qiang He

Journal: Mol Med Rep

PubMed ID: 34165172

Doi: 10.3892/mmr.2021.12231

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