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Effects of the I559P gp41 Change on the Conformation and Function of the Human Immunodeficiency Virus (HIV-1) Membrane Envelope Glycoprotein Trimer.

PLoS One.. 2015-04; 
Alsahafi N, Debbeche O, Sodroski J, Finzi A. Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, Massachusetts, 02115, United States of America
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Mutagenesis Services ...Site-directed mutagenesis. The sequence of full-length clade A HIV-1 BG505 Env [47] was codon-optimized (<b>genscript</b>) and cloned into the expression plasmid pcDNA3.1. Mutations were introduced individually or in combination into this plasmid, pcDNA3.1-BG505.... Get A Quote

摘要

The mature human immunodeficiency virus (HIV-1) envelope glycoprotein (Env) trimer is produced by proteolytic cleavage of a precursor and consists of three gp120 exterior and three gp41 transmembrane subunits. The metastable Env complex is induced to undergo conformational changes required for virus entry by the binding of gp120 to the receptors, CD4 and CCR5/CXCR4. An isoleucine-to-proline change (I559P) in the gp41 ectodomain has been used to stabilize soluble forms of HIV-1 Env trimers for structural characterization and for use as immunogens. In the native membrane-anchored HIV-1BG505 Env, the I559P change modestly decreased proteolytic maturation, increased the non-covalent association of gp120 with the En... More

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