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Custom Vector Construction> | … The laccase coding sequence (GenBank HQ857207), synthesized by Genscript (Piscataway, USA) from pET-22b(+) (Novagen, Darmstadt, Germany) vector was amplified in the PCR reaction (94 °C for 4 min, 1 cycle; 94 °C 1 min/50 °C 1 min/72 °C for 2.45 min, 30 cycles; 72 °C … | Get A Quote |
High amounts of toxic textile dyes are released into the environment due to coloring and wastewaters treatment processes' inefficiency. To remove dyes from the environment and wastewaters, researchers focused on applying immobilized enzymes due to mild reaction conditions and enzyme nontoxicity. Laccases are oxidases with wide substrate specificity, capable of degradation of many different dye types. Laccase from Streptomyces cyaneus was expressed on the surface of Saccharomyces cerevisiae EBY100 cells. The specific activity of surface-displayed laccase was increased by toluene-induced lysis to 3.1 U/g of cell walls. For cell wall laccase immobilization within hydrogel beads, alginate was modified by dopamine u... More