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Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization

Int J Biol Macromol. 2021-04; 
Nikolina Popović, Dunja Pržulj, Maja Mladenović, Olivera Prodanović, Selin Ece, Karla Ilić Đurđić, Raluca Ostafe, Rainer Fischer, Radivoje Prodanović
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Custom Vector Construction … The laccase coding sequence (GenBank HQ857207), synthesized by Genscript (Piscataway, USA) from pET-22b(+) (Novagen, Darmstadt, Germany) vector was amplified in the PCR reaction (94 °C for 4 min, 1 cycle; 94 °C 1 min/50 °C 1 min/72 °C for 2.45 min, 30 cycles; 72 °C … Get A Quote

摘要

High amounts of toxic textile dyes are released into the environment due to coloring and wastewaters treatment processes' inefficiency. To remove dyes from the environment and wastewaters, researchers focused on applying immobilized enzymes due to mild reaction conditions and enzyme nontoxicity. Laccases are oxidases with wide substrate specificity, capable of degradation of many different dye types. Laccase from Streptomyces cyaneus was expressed on the surface of Saccharomyces cerevisiae EBY100 cells. The specific activity of surface-displayed laccase was increased by toluene-induced lysis to 3.1 U/g of cell walls. For cell wall laccase immobilization within hydrogel beads, alginate was modified by dopamine u... More

关键词

Dopamine, Laccase, Yeast surface display